Synthesis of cephalosporin C from sulfate by mutants of Cephalosporium acremonium.
نویسندگان
چکیده
The innate ability of Cephalosporium acremonium to use methionine preferentially over sulfate for synthesis of cephalosporin C can be influenced through mutation. Mutants of C. acremonium with altered capacity to utilize sulfate for synthesis of antibiotic were isolated and partially characterized with respect to the uptake of sulfate and the regulation of arylsulfatase.
منابع مشابه
Optimization of Cephalosporin C Production by Cephalosporium Acremonium C-10 Using Stationary Culture
The present study is concerned with the production of cephalosporin C by Cephalosporium acremonium C-10. Two culture media were tested for the production of antibiotic. Among these culture media M1 containing sucrose, ammonium acetate, cornstarch, sugar cane molasses, soybean meal, calcium carbonate, calcium sulphate and methyl oleate gave optimum production of cephalosporin C. The cultural con...
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To examine microbiological ring expansion of penicillin N to a cephalosporin, we obtained five mutants of Cephalosporium acremonium blocked in beta-lactam antibiotic biosynthesis from 2500 survivors of mutagenesis. In submerged fermentation, mutants M-0198, M-0199, and M-2351 produced no beta-lactam antibiotic (type A), whereas mutants M-1443 and M-1836 formed penicillin N but not cephalosporin...
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The origin of desacetylcephalosporin C in cephalosporin C fermentation broths was investigated. Esterase activity was detected in cell-free extracts of Cephalosporium acremonium, but these extracts failed to deesterify cephalosporin C. When cephalosporin C was added to sterile and inoculated fermentation media, the antibiotic decayed at nearly identical rates. The formation of desacetylcephalos...
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Cephalosporium acremonium was cultivated in fermentation medium containing sucrose or methyl oleate as a carbon source for cephalosporin C production. The level of antibiotic production was 48 g of cephalosporin C per liter under optimum conditions when methyl oleate was used. The C(18:1) (oleic acid) methyl ester appeared to be utilized faster than the C(18:2) (linoleic acid) methyl ester in f...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 4 4 شماره
صفحات -
تاریخ انتشار 1973